《植物生理学报》 2011, 47(4): 409-414

一种高效获取基因5′末端的RACE方法

罗聪¹, 何新华^1,2,*, 陈虎¹, 韦泳丽¹, 李明娟¹

1广西大学农学院, 南宁530004; 2广西作物遗传改良与生物技术重点实验室, 南宁530007

通信作者：何新华；E-mail: honest66222@163.com；Tel: 0771-3270184

摘　要：

RACE技术是一种快速高效克隆基因5′末端和3′末端的方法, 是获取基因全长的主要手段之一, 但是RACE技术本身 也存在一些缺点。我们在前人改良的RACE技术基础上进一步优化RACE技术, 获得了一种操作简单、快速、高效、成本 低廉的改良RACE方法, 该方法适合于大量基因5′末端的获取, 可以在普通实验室推广应用。

关键词：RACE; TdT; 方法改良

收稿：2011-01-10   修定：2011-02-17

资助：广西高等学校优秀人才资助计划项目(桂教人201065)、广西大学博士后启动基金(20090042)、广西自然科学基金 (2011GXNSFA018115)和广西教育厅研究生创新计划项目 (105931001011)。

A High-Efficient Method of RACE Technique for Obtaining the Gene 5′ End

LUO Cong¹, HE Xin-Hua^1,2,*, CHEN Hu¹, WEI Yong-Li¹, LI Ming-Juan¹

¹College of Agriculture, Guangxi University, Nanning 530004, China;²Guangxi Crop Genetic Improvement and Biotechnology Lab, Nanning 530007, China

Corresponding author: HE Xin-Hua; E-mail: honest66222@163.com; Tel: 0771-3270184

Abstract:

RACE technique is a rapid and effective method for cloning gene 5′ end and 3′ end, and one of main methods to obtain full-length genes. However, RACE technique has its own shortcomings. We further improved the previous RACE protocols and obtained a modified RACE technique which is simple, rapid, high-efficient and low-cost. Our modified RACE technique is suitable for obtaining large number of genes 5′ end and can be used in ordinary laboratories.

Key words: RACE; TdT; modified method